US EPA

8315346 

Journal Article 

Invitro evaluation of antidiabetic potential and phytochemical profile of Psoralea corylifolia seeds 

Suhashini, R; Sindhu, S; Sagadevan, E 

2014 

0 

International Journal of Pharmacognosy and Phytochemical Research
ISSN: 0975-4873 

International Journal of Pharmacognosy and Phytochemical Research 

6 

2 

414-419 

English 

Diabetes is a metabolic disorder that occurs due to poor secretion of insulin by pancreas. Insulin helps to metabolize blood sugar. Hence this results in high glucose level in blood. Presently, diabetes is considered as a big killer and is among the most significant diseases in the developed world. The incidence of diabetes is increasing every day and this indicates the increasing need for the treatment of diabetes. The blood glucose level can be regulated by various mechanisms. In vitro antidiabetic assays such as glucose uptake by yeast cells, glucose diffusion assay, alpha amylase inhibition assay and glycosylation of hemoglobin assay are performed. Using these assays, the antidiabetic activity of the plant Psoralea corylifolia is determined. Qualitative and quantitative phytochemical analysis reveals all the bioactive compounds present in the crude extract of the plant. Our results show that methanolic extract has an effective activity when compared to ethyl acetate and hexane extracts. Hence methanolic extract of Psoralea corylifolia possess higher antidiabetic activity. The compounds which contribute to its antidiabetic activity were identified, characterized and separated by thin layer chromatography. MTT assay for L6 cell lines was performed and was found that the plant extract showed a minimum toxicity. 

Antidiabetic activity; Cell lines; Diabetes; Extract; Glucose; In vitro assay