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HERO ID
8316073
Reference Type
Journal Article
Title
RNA fluorescence in situ hybridization using 3-cyanovinylcarbazole modified oligodeoxyribonucleotides as photo-cross-linkable probes
Author(s)
Fujimoto, K; Toyosato, Kei; Nakamura, S; Sakamoto, T; ,
Year
2016
Is Peer Reviewed?
Yes
Journal
Bioorganic & Medicinal Chemistry Letters
ISSN:
0960-894X
EISSN:
1464-3405
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Location
OXFORD
Page Numbers
5312-5314
PMID
27680586
DOI
10.1016/j.bmcl.2016.09.035
Web of Science Id
WOS:000389015300033
URL
https://linkinghub.elsevier.com/retrieve/pii/S0960894X1630974X
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Abstract
Photo-cross-linkable fluorescent oligodeoxyribonucleotides having 3-cyanovinylcarbazole nucleoside were applied to fluorescence in situ hybridization (FISH) based 16S rRNA detection in Escherichia coli cells. As the photo-cross-linked probe/rRNA hybrid was stable under the denaturing condition, decrease of the fluorescence signal through the washing process was prevented. The thermally irreversible hybridization property also enabled stable hybridization with the structured region on the target RNA, and facilitated design of the sequence for the FISH probe. Further development of the method might contribute to quantitative and stable FISH staining. (C) 2016 Elsevier Ltd. All rights reserved.
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