US EPA

8316197 

Journal Article 

Chemical composition and characterization studies of cassia auriculata flower extract 

Soundharajan, SR; Ponnusamy, RD 

2014 

1 

International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 

International Journal of Pharmacy and Pharmaceutical Science 

6 

7 

327-330 

English 

Objectives: The present study, aimed to determine and characterize the chemical constituents of Cassia auriculata flower extract by qualitative, quantitative and analytical techniques. Methods: Preliminary Phytochemical, total flavonoid and phenol content was determined in the methanolic extract of C.auriculata (CAFMEt) using standard methods. C-18 silica gel based column chromatography was used to purify CAFMEt using n-hexane, ethyl acetate and methanol and fraction identified by thin layer chromatography. GC-MS and FT-IR techniques used to characterize the lead fraction. Results: CAFMEt showed the presence of flavonoid and Phenols in a significant amount. Three fractions was collected from column chromatography viz., Fraction 1-3 (n-hexane: yellow) was 2.5mg, (ethyl acetate: light orange) 1.8mg and (methanol: light green) 5.67mg respectively. TLC indicated n-hexane has higher refractive factors 0.457 at yellow band and ethyl acetate fraction has 0.329 at light orange band. 14 chemical constituents were identified by GC-MS included alkanes, alcohol, esters and hydrocarbons. The major peak showed the presence of 4-(4-methylphenoxy) phenol at 22.53%. Infra red spectra revealed the presence of phenolic groups in hexane fraction. Conclusion: Further studies will be carried out the pharmacological potential of n-hexane fractions of flowers of C.auriculata. 

Ethyl acetate; Flavonoid; FT-IR; N-hexane; Phenol