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8317126 
Journal Article 
Isolation and identification of Eisenia Foetida associated Pseudomonas Aeruginosa and its control 
Shahnawaz, A; Andleeb, S; Ali, S 
2011 
0 
Punjab University Journal of Zoology
ISSN: 0079-8045 
Punjab University - Department of Zoology 
26 
1 
31-44 
English 
Earthworm increases the soil fertility and is often referred to as a farmer's friend. The bacterial pathogen Pseudomonas (P) aeruginosa associated with the earthworm, penetrate into the body, cause diseases to the host and leads to the opportunistic infection. Eisenia (E) foetida collected from the local soil and P. aeruginosa spp. were isolated. The bacterial strain was confirmed through morphological and biochemical tests. In order to control the pathogenicity of P. aeruginosa, we used chloroform and isoamylalcohol extracts of medicinal plant including Cinnnamomum zylanicum, Cuminum cyminum, Syzygium aromaticum, Curcuma long Linn, Trachyspermum ammi as well as the n-Hexane, chloroform, ethanol, methanol and ethyl acetate extracts of Momordica charantia were investigated. On the other hand antibiotic sensitivity showed that isolated P. aeruginosa was highly sensitive to streptomycin, gentamycin and ciproflaxin. It was concluded that the extracts of these medicinal plants have considerable effect on the bacterial pathogens. Isolation and purification of different phytochemicals may further yield significant antibacterial agents. © 2011, Dept. Zool., P.U., Lahore, Pakistan. 
Antibiotic sensitivity; Bacteria; Earthworm; Momordica charantia