US EPA

8317265 

Journal Article 

Anti-oxidant and anti-inflammatory activities of Tetrastigma sulcatum (Law.) gamble leaf extract and its fractions 

Waghole, RJ; Misar, AV; Mujumdar, AM; Naik, DG 

2015 

0 

International Journal of Pharmacognosy and Phytochemical Research
ISSN: 0975-4873 

International Journal of Pharmacognosy and Phytochemical Research 

7 

5 

896-902 

English 

Hitherto unknown in vitro anti-oxidant and anti-inflammatory activities of crude ethanolic extract of leaf of Tetrastigma sulcatum, commonly known wild grapes, and its fractions were demonstrated. The total crude ethanolic extract as well as its hexane, toluene and ethyl acetate fractions were screened for antioxidant activity. The antioxidant activities were determined by DPPH, β-Carotene bleaching, reducing power assays. Total phenolic contents (TPC) of extracts and fractions ranged from 10 to 60 mg of GAE/g dry weight and correlated well with the activities observed. Screenings of the samples using TPA induced mouse ear oedema model indicated the anti-inflammatory activity of the total ethanolic extract. Interestingly the toluene fraction showed a better activity while activity of the hexane fraction was slightly inferior. The ethyl acetate fraction and the residue left after fractionation did not show anti-inflammatory properties. Details of the screening are reported. © 2015, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved. 

Anti-oxidant; Fractions; Local anti-inflammatory activity; Tetrastigma sulcatum leaf extract