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8318031 
Journal Article 
Estimation of Gemfibrozil in tablet dosage form by HPTLC method 
Vikas Parikh, C; Karkhanis, VV 
2011 
0 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
2 
3 
889-898 
English 
A simple, precise, accurate and rapid high-performance thin-layer chromatographic method has been developed for the estimation of Gemfibrozil in tablet dosage forms. The stationary phase used was pre-coated silica gel 60 F 254. The mobile phase used was a mixture of Toluene; Hexane; Ethyl Acetate; Glacial acetic acid [6:2:2:0.1 v/v/v/v]. The detection of spots was carried out at 276 nm. The calibration curve was found to be linear between 1000ng/spot to 5000ng/spot for Gemfibrozil. The limit of detection and the limit of quantification for Gemfibrozil were found to be 176.40ng/spot and 534.54ng/spot. The method was found to be accurate with 99.71-100.09 % recovery and precise with %RSD 0.26-0.53 for intra-day [n=3] and % RSD 0.20 - 0.44 for inter-day [n=3] for Gemfibrozil. The proposed method can be successfully used to determine the drug content of marketed formulation. The developed method of HPTLC for Gemfibrozil was also validated by performing different validation parameters. The result demonstrated that the procedure is accurate, precise and reproducible, suitably applied for the determination of Gemfibrozil in different dosage forms. 
Gemfibrozil [GEM]; HPTLC; Tablet; U.V. Spectrophotometry; Validation