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8318939 
Journal Article 
A simple and efficient method for extraction and quantification of capsaicin from pepper fruits through high performance thin layer chromatography 
Das, A; Kundu, S; Ghosh, B 
2015 
0 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
Research Journal of Pharmaceutical, Biological and Chemical Sciences 
6 
1 
606-612 
English 
A simple and efficient method for extraction and quantification of capsaicin using high performance thin layer chromatographic (HPTLC) was developed and validated from seven different types of Capsicum annuum fruit. The crude extracts were subjected to TLC for the qualitative and quantitative examination for the capsaicin. The chromatographic separation was carried out on precoated silica gel plate using Chloroform: Methanol: Acetic acid: Hexane (2.85: 0.15: 0.15: 1, v/v/v) as mobile phase and densitometric analysis was carried out in absorbance mode at 282 nm. The mobile phase gave well defined peaks of capsaicin at Rf value of 0.78. The linear regression analysis data for the calibration plots using capsaicin standard showed good linear relationship with regression coefficient (R2) of 0.985 for height and 0.995 for area, respectively; in the concentration range of 1-5 μg/spot. Among the fourteen different solvent, the ethanol produce maximum extraction efficiency of capsaicin. The proposed HPTLC method can be applied for robust identification and quantitative determination of capsaicin from different types of pepper fruits. 
Capsaicin; Capsicum annuum; HPTLC