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8319806 
Journal Article 
Bioactive potential 6f Tonna galea (Linne. 1758) from Gulf of Mannar 
Santhi, V; Sivakumar, V; Thilaga, RD; Borgia, VJF 
2011 
5 
3 
130-135 
English 
Crude methanol was prepared from whole Tonna galea (Tonnidae) and fractionated by Hexane: Chloroform (Fl), Chloroform (F2), Benzene (F3), Benzene: Methanol (F4) and methanol (F5) solvents were analyzed for antimicrobial activity using agar well diffusion technique against several bacterial pathogens. Minimal inhibitory concentrations were then determined for the most potent fraction. To identify the compound responsible for antibacterial activity, the potent fraction was subjected to GC-MS analysis. The broadest antibacterial activity was noted in fraction 1 and fraction 5 The highest activity was exhibited against Vibrio cholerae (0139) in the crude extract. The column purified Hexane: Chloroform fraction showed higher activity against Vibrio cholerae 0139 (8mm) and maximum inhibition zone was exhibited against Aeromonas hydrophila (10mm). These promising results confirms the presence of Benzenedicarboxylic acid. Mono (2-ethyle xyl) ester, squalene and methyl 3-bromol-l- adamantaneacte by GC-Ms may be related to mollusc defense mechanism and antimicrobial activity against these pathogens. © IDOSI Publications, 2011. 
Antibacterial; Gulf of Mannar; Mollusc