US EPA

8320358 

Journal Article 

In-vitro antibacterial activity, phytochemical screening and characterization of morinda tinctoria root extracts 

Gopalan, S; Kulanthai, K; Sathasivam, G 

2015 

0 

Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 

Research Journal of Pharmaceutical, Biological and Chemical Sciences 

6 

1 

879-888 

English 

The objective of the present investigate to evaluate the presence of phytochemical constituents, characterization and antibacterial activity of different extracts of roots of Morinda Tinctoria. The extraction was done with the series of solvents like hexane, ethyl acetate and methanol with increasing polarity using soxhlet apparatus. The phytochemical analysis was done by using the slandered procedure. The Plant extracts were characterized by UV-vis, FT-IR spectrum. These extracts were tested for antibacterial activity against four human pathogens viz., Streptococcus aureus, Bacillus subtilis, Pseudomonas Auregenosa, Escherichia coli. The results revealed that the roots extracts contain phytochemical constituents like Alkaloids, Phytosterols, Flavonoids, Phenols and Triterpenes. Ethyl acetate extract shows more effective against the gram-positive organisms as well as gram-negative organism, followed by hexane and methanol extracts. The UV-vis spectrum profile showed different peaks ranging from 232nm to 372nm with different absorption respectively. The presence of active compounds was identified by FT-IR. The present study concludes that the different extract of M. Tinctoria roots contains good antibacterial activity against tested microorganisms. The present study concluded that M. Tinctoria roots extracts can be investigated to discover the bioactive natural products that may present as leads in the improvement of new pharmaceuticals. 

antibacterial activity; FT-IR; Morinda Tinctoria roots; UV-vis