US EPA

8323462 

Journal Article 

Antibacterial properties of limau kasturi (C. Microcarpa) peels extract 

Rubiatul, AS; Nor Helya, IK; Zarina, Z; Dachyar, A; Nurulain, HA 

2015 

Yes 

Advances in Environmental Biology
ISSN: 1995-0756 

American-Eurasian Network for Scientific Information 

9 

19 

5-9 

English 

Citrus microcarpa or Citrus mitis, also known as ‘limaukasturi’ is an edible plant and have potential as antibacterial agent. The presence of bioactive compound in limaukasturi peels extract have possessed antibacterial property by killing or inhibits the growth of pathogenic bacterial. In this research, limaukasturi peels were dried and extracted using soxhlet extraction method. The solvents used for the extraction were methanol, ethanol, acetone, hexane and water. The antibacterial effect of limaukasturi peels extract was evaluated on Gram-positive bacteria like Bacillus subtilisand Streptococcus spp., and Gram-negative bacteria such as Escherichia coli and Salmonella spp. The extracted products were subjected to antibacterial susceptibility assay by agar well-diffusion method with measurement diameter of inhibition zone around the extracts. From the result, methanol extracts showed the highest inhibition zone compared to other solvent extracts. The zone of inhibition of methanol extracts on E. coli, Salmonella spp., B. subtilis, and Streptococcus spp. were19 mm, 17 mm,13 mm and 12 mm, respectively. Overall, the bacterial aresusceptible and moderately susceptibleto methanol extracts whereas the other extracts of ethanol, acetone, hexane and water showed low inhibition and no inhibition effects towards four bacterial tested. Thus, the results obtained in the present study suggest that methanol extracts of limaukasturi peels can be used for further study in treating diseases caused by the test organisms. © 2015 AENSI Publisher All rights reserved. 

Agarwell diffusion method; Antibacterial properties; Limau kasturi; Peels; Soxhlet extraction