Kudle, KR; Kudle, MR; Shirisha, M; Pratap Rudra, MP
In this study we synthesized Silver nano Particles and screened Phytochemical and antimicrobial activities. Fresh aqueous leaf, Flower, Root extracts of Grandiflora (Nicotiana Alata) were used for synthesis of silver nanoparticles. The biosynthesized AgNPs were characterized by UV-Spectra, FTRI, Transmission Electron Microscope (TEM). Good activity against all the bacteria strains in comparison with Ampicillin. Grandiflora (Nicotiana alata) leaves, flowers, root of the plants were extracted with Hexane Methanol, Pet ether. Methanol, Pet. ether extracts of leaf and flower, Root were tested quantitatively for phytoconstituents. AgNPs bounded protein estimation Lowaryâs method. The Phytochemical properties of the leaf, flower, Root of Grandiflora (Nicotiana alata) were also done in present investigation. Absorption spectrum at different wavelengths ranging from 400-500 nm revealed a peak of λ max at 430 nm. The TEM images clearly reveals that shape and size of silver nanoparticles was 15-30 nm with spherical shape. TLC Plate of Plant leaf Hexane extract contain not clearly separation componds, Pet. Ether Extract contain only two spots observed (Rf Value 0.35, 0.6cm) and methanol extract of Grandiflora (Nicotiana Alata) plate separation of phytochemical compounds was separation five clear spots observed in methanol extract(Rf Value 0.45, 0.50, 0.7, 0.8, 1.0cm). Results showed the presence of phytoconstituents Alkaloids, tannins, phenolic compounds, proteins, terpenoids and saponins. The Antibacterial activities of the AgNPs were evaluated by silver nano particles. However, the natural products profile and consequently the bioactivity is known to vary with the climate and geographic location of the plants. The present study also correlates with the results of previously reported with little variations. Nanoparticles are important due to their simple experimental procedure and eco-friendliness. © 2015, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.