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8325219 
Journal Article 
Variability of antioxidant properties and identification of phenolic contents by HPLC-DAD in different organs of acacia albida and acacia raddiana 
Karoune, S; Kechebar, MSA; Djellouli, A; Belhamra, M; Rahmoune, C; Ksouri, R 
2016 
0 
International Journal of Pharmacognosy and Phytochemical Research
ISSN: 0975-4873 
International Journal of Pharmacognosy and Phytochemical Research 
8 
5 
701-709 
English 
The mainly aims of this work is to study the interspecific variability of polyphenol contents and anti-radical activity of two species, Acacia albida and Acacia raddiana in order to select the best species in both respects, quantitative (phenolic content) and qualitative (antioxidant activity). Initially we performed a sequential extraction with four solvents: Hexane, Chloroform, Ethanol and Water on three organs leaves, fruit and bark, collected on field in the Tindouf region. The eighteen extracts obtained were assayed for total polyphenols by the Folin-Ciocalteu method and an evaluation of the antioxidant activity by testing the free radical DPPH. Results showed significant differences between organs of both species for the three extracting solvents as well as for the polyphenol contents and antioxidant properties. The ethanol extracts of Acacia albida exhibit the best results of total polyphenol concentration with 100.94 and 59.50 mg GAE.g-1DW, respectively for the leaves and bark. The IC50 values of the DPPH test is in favor of leaves and bark of Acacia albida for both aqueous and ethanolic extracts. For the ethanolic extract we recorded IC50 values of 28 and 26 μg.ml-1 for leaves and bark, while the aqueous extract exhibited the values of 22.5 and 29 μg.ml-1 for leaves and bark, respectively. Both extracts were analyzed by high performance liquid chromatography (HPLC-DAD). These results allow us to conclude that the Acacia albida is the most active species in comparison to Acacia raddiana and the ethanol and aqueous extracts of leaves and bark have achieved the best values. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved. 
Acacia albida; Acacia raddiana; Bark; DPPH; Leaves; Polyphenol