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8325758 
Journal Article 
Effect of leek (Allium ampeloprasum L.) extract on biotransformation enzymes and innate immunity of catfish (Clarias gariepinus) exposed to benzo[a]pyrene 
Abbas, WT; Awada, E 
2016 
0 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
Research Journal of Pharmaceutical, Biological and Chemical Sciences 
7 
6 
2210-2223 
English 
Aquaculture is subjected to various toxicants which affect the health status ending with dramatically lost production. The study investigated the prospective effect of Leek extract (Allium ampeloprasum L.) on detoxification and innate immuno response of catfish (Clarias gariepinus) exposed to benzo[a]pyrene (BaP). Fish were divided into 4 groups before being injected with 0 (control), 5, 50, 500 mg/kg of leek extract (dissolved in Dimethyl sulfoxid). After 2 weeks, fish were exposed to 1mg/L of BaP for 24 h and sampling was done before and after exposure. Biotransformation enzymes (cytochrome P4501A1 (CYP4501A1), Ethoxyresorufin-O-Deethylase (EROD) and Glutathione-S-transferase (GSH) activities), BaP metabolites in bile and innate immune parameters (antiproteases, myeloperoxidase, total protein, albumin and globulin) were examined. Results demonstrated significant induction in EROD and GSH activities in all fish groups injected leek extract, while after exposure to BaP, their induction recorded the highest values in the low dose group (5mg/Kg). Moreover, CYP450 content showed no significant differences before and after exposure to BaP. No metabolite residues were detected in fish bile in both control and treated groups after BaP exposure using GC-MASS analysis. Furthermore, all doses of leeks showed enhancement in all examined innate immune parameters. However after exposure to BaP, only 5 mg/kg dose demonstrated ability to resist the toxic effect of BaP and enhanced immune parameters. To conclude, the injection of catfish with low dose of leek extract may contribute in protecting fish from harmful effect of BaP through enhancement biotransformation and immune systems. 
Allium ampeloprasum L.; Clarias gariepinus; Immunity