US EPA

8325844 

Journal Article 

Qualitative phytochemical screening and in vitro antioxidant activity of hybanthus enneaspermus 

Anupa, MP; Chinju, S; Murugan, M 

2016 

0 

International Journal of Pharmacognosy and Phytochemical Research
ISSN: 0975-4873 

International Journal of Pharmacognosy and Phytochemical Research 

8 

6 

1046-1049 

English 

The present study was aimed to determine the phytochemical constituents and antioxidant activity of different extracts of Hybanthus enneaspermus (L). Dried plant leaf was extracted with different solvents such as hexane, chloroform, ethyl acetate, ethanol and distilled water. Phytochemical screening was performed with standard protocols and this study showed the presence of flavonoids, terpenoids, tannins, phenol and saponin. Also the ethanol and aqueous extract contained more phytochemicals. Antioxidant activity of the ethanol extract was determined by DPPH assay, reducing power and hydrogen peroxide radical scavenging assay. The results showed that, the maximum radical scavenging activity in DPPH activity 24.32% and the standard was 45.41% at 60 mg/ml of concentration. The reducing power assay exhibited maximum absorbance of 1.038 at 60 mg/ml of concentration and in the hydrogen peroxide radical scavenging activity, the percentage of inhibition was 35.11%. Thus the ethanolic leaf extract of H. enneaspermus has a significant antioxidant activity and used as a better source of natural antioxidants which might be helpful in preventing the progress of oxidative stress. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved. 

Antioxidant activity; Hybanthus enneaspermus; Phytochemicals