Health & Environmental Research Online (HERO)


Print Feedback Export to File
8327046 
Journal Article 
Development and Validation of RP-HPLC method for simultaneous estimation of combination of eperisone hydrochloride and naproxen sodium 
Divekar, RB; Kulkarni, AA; Deshpande, PB; Mathdevru, BV; Ram, B; Bhalerao, KS 
2016 
Scholars Research Library 
8 
17 
106-114 
English 
A simple, rapid and precise Reverse Phase High Performance Liquid Chromatographic method was developed for simultaneous estimation of Eperisone hydrochloride and Naproxen sodium in newly developed tablet dosage form by reverse phase HiQSil C-18 column (250 mm, 4.6 mm, and 5 μm). The sample was analyzed using: Acetonitrile: 10mM n-hexane sulfonic acid buffer (70:30 v/v), as a mobile phase at a flow rate of 1.0 mL/min. and detection at 250 nm. The retention time for Eperisone hydrochloride and Naproxen sodium was found to be 2.77 min and 9.56 min, respectively. The linearity of developed method was achieved in the range of 1-10 μg/mL for Eperisone hydrochloride and 1-25 μg/mL for Naproxen sodium. The method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation, robustness and ruggedness as per ICH guidelines. 
Eperisone hydrochloride (EPE); Naproxen sodium (NAP); RP-HPLC; Validation