US EPA

8327500 

Journal Article 

Antioxidant capacities of various grains extracts of three kinds of rice grown in Central Java-Indonesia 

Fidrianny, I; Puspitaningrum, DA; Ruslan, K 

2016 

0 

International Journal of Pharmacognosy and Phytochemical Research
ISSN: 0975-4873 

International Journal of Pharmacognosy and Phytochemical Research 

8 

6 

997-1002 

English 

The goals of this research were to determine antioxidant activity from different polarities grains extract of three kinds of rice using two methods of antioxidant testing which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) and correlation of total phenolic, flavonoid and carotenoid content with their IC50 of DPPH and IC50 of FRAP antioxidant activities. Extraction was carried out by reflux using different polarities solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH and FRAP assays, determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with IC50 of DPPH scavenging activities and EC50 of FRAP capacities were analyzed by Pearson’s method. Ethyl acetate and ethanolic extracts of black rice and red rice grains were very strong antioxidant, using DPPH assays. Phenolic compounds in black rice and red rice grains extracts were the major contributor in IC50 of DPPH scavenging activity and EC50 of FRAP capacity. Grains extract of black rice, red rice and white rice had linear result in DPPH and FRAP assays. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved. 

Antioxidant; DPPH; FRAP; Grains; Rice; Three kinds