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8327715 
Journal Article 
Evaluation of antioxidant and antiproliferative activities of Nannochloropsis gaditana extracts 
Mekdade, L; Hamed, MBB; El-Kebir, FZ; Ayad, SMEAA 
2016 
0 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
Research Journal of Pharmaceutical, Biological and Chemical Sciences 
7 
3 
904-913 
English 
To identify a new source of natural antioxidants and anticancer agents from marine microalgae, we investigated the evaluation of phenolic compounds in Nannochloropsis gaditana extracts namely, acetonic, methanolic, dichloromethanic, hexanic and aqueous extracts. The antioxidant activity of these extracts was evaluated by two methods: The scavenging of DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical and the ferric reducing power. The antiproliferative activity was also assessed on A549 (Human lung cancer cell line) by MTT assay. The results revealed that the aqueous extract and the methanolic extract had the highest amount of phenolic compounds and the highest ferric reducing power. In DPPH assay, the aqueous extract and the acetonic extract exhibited the highest antioxidant activity. The acetonic extract showed the best effect on A549 cell line. The obtained results suggest that the antioxidant activity of N.gaditana extracts is not only due to the presence of phenolic compounds, but also due to the presence of other molecules that act as scavengers of free radicals via the hydrogen atom transfer and the electron transfer. Moreover, others molecules with an anticancer effect must be present in the acetonic extract, giving it an anti-proliferative activity on A549 cell line. © 2010 RJPBCS. 
Antiproliferative activity; DPPH; Nannochloropsis gaditana; Phenols; Reducing power