US EPA

8328384 

Journal Article 

Partially Deactivated Silica Gel Columns in Chromatography. Chromatographic Behavior of Benzo[a]pyrene 

Cahnmann, HJ 

1957 

Yes 

Analytical Chemistry
ISSN: 0003-2700
EISSN: 1520-6882 

29 

9 

1307-1311 

English 

10.1021/ac60129a018 

Various activity grades of silica gel were prepared by the controlled deactivation of activated silica gel with known amounts of water. Moderately deactivated silica gel gave better defined chromatographic zones than a nondeactivated gel in the fractionation of many polycyclic aromatic hydrocarbons. The shapes of the elution curves of benzo [a]pyrene, which was chosen as a typical example, show that by the gradual addition of water to activated silica gel an optimal degree of deactivation is reached, beyond which further deactivation yields poorer adsorbents. RF values of benzo[a]pyrene for various activity grades of silica gel are presented and the advantages offered by the use of a partially deactivated silica gel are discussed. © 1957, American Chemical Society. All rights reserved.