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Journal Article 
Phytochemical screening and antimicrobial potentials of Alangium salvifolium and Piper longum against multi-drug resistant bacteria from clinical isolates 
Dahiya, P; Purkayastha, S 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
The antimicrobial activities of various solvent extracts of Alangium salviifolium and Piper longum were evaluated against clinically proved multidrug resistant bacteria (Methicillin-resistant Staphylococcus aureus, Enterococcus sp., Klebsiella sp., Escherichia coli, Pseudomonas aeruginosa and Acinetobacter sp. and reference strains of bacteria (Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922) by using agar well diffusion assay. The patterns of inhibition varied with the plant extract, the solvent used for extraction, and the organism tested. Methicillin- resistant Staphylococcus aureus (MRSA) and Enterococcus sp. were the most inhibited microorganisms. The highest antimicrobial potentials were observed for the hexane and acetone extracts of A. salviifolium and P. longum, displaying maximum inhibitory zone of 18 mm against Enterococcus sp. Acinetobacter sp. was susceptible only to hexane extract of A. salviifolium. However, aqueous extracts did not present any antibacterial activity. Phytochemical screening showed that all the extracts contain alkaloids and reducing sugars while anthraquinones were absent in all the extracts tested. Hexane and acetone extracts were separated using TLC and relative mobilities of bioactive components showing significant inhibitory zones against S. aureus MRSA and Enterococcus sp. were determined by bioautography agar overlay assay. 
Agar well diffusion; Multi-drug resistant; Phytochemical analysis; TLC bioautography