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8332461 
Journal Article 
Pivotal role of plant growth regulators in clonal propogation of Melia azedarach L 
Sen, A; Batra, A; Rao, DV 
2010 
5 
2 
43-49 
English 
A procedure has been standardized using a diverse array of plant growth regulators for in vitro regeneration of 'paradise tree' (Melia azedarach L., Meliaceae) from in vitro nodal explants. The immature seeds were allowed to germinate on filter bridges having sterilized distilled water. After 3 weeks, seeds were germinated and the in vitro grown seedlings were then used as source of explants. BAP (1.2 mg/l) has been found to be optimum for shoot initiation when in vitro nodal sections were inoculated. For further growth and multiplication, they were then transferred to fresh MS medium having optimized BAP (1.2 mg/l) along with IAA (0.1 mg/l), adenine sulphate (9.0mg/l), ammonium sulphate (200 mg/l) and potassium sulphate (100 mg/l). Repeated and periodic sub culturing yielded better growth and multiplication with 90% shoot formation. In vitro rooting was obtained on half strength MS medium supplemented with IBA (1.0 mg/l). Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived under natural conditions after transplantation. 
Clonal Propagation; Immature seeds; In vitro rooting; Melia azedarach L.; Multiple shoots