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8332735 
Journal Article 
32P-Postlabeling e poliacrilamide gel elettroforesi: Confronto e applicazione delle due tecniche per il rilevamento degli addotti al DNA 
Pappalardi, B; Policastro, MV; Chiumarulo, P; Assennato, G; Corsi, P 
2006 
Yes 
Giornale Italiano di Medicina del Lavoro ed Ergonomia
ISSN: 1592-7830 
28 
3 SUPPL. 
16-17 
Italian 
BACKGROUND. 32P-Postlabeling analysis is a powerful technique to detect DNA adducts induced by endogenous and exogenous mutagens or carcinogens. OBJECTIVES. In the present study two techniques were applied to the detection of BPDE-DNA adducts: a newly developed Postlabeling coupled with nondenaturing 20% polyacrylamide gel electrophoresis, 32P-Postlabeling/PAGE and 32P-Postlabeling/TLC. The aim was to consider PAGE valuable and widely useful also for biomonitoring study of exposure to PAH. METHODS. Polyethylenimine (PEI)-cellulose TLC plates were used to separate 32P-labeled adducts two-dimensionally under several different buffer conditions; the same DNA samples were applied on nondenaturing 20% polyacrylamide gel electrophoresis for 32P-Postlabeling/PAGE. CONCLUSIONS. While the major advantage of this technique is that many DNA samples can be analysed on the same gel and resolved in a few hours nevertheless it showed lower recoveries of BPDE-DNA adducts compared to TLC. 
32P-Postlabeling/PAGE; Addotti BPDE; TLC