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8333639 
Journal Article 
Preparative HPLC for analysis of mineralocorticoids in human plasma 
Lejeune-Lenain, C; Kina, S; Bosson, D 
1987 
Yes 
Chromatographia
ISSN: 0009-5893
EISSN: 1612-1112 
Springer-Verlag 
WIESBADEN 1 
24 
333-338 
English 
A method has been developed to measure simultaneously in human plasma the mineralocorticoids and glucocorticoids involved in mineralocorticoid excess. Briefly: plasma extracts were chromatographied in a fully-automated procedure on a lichrospher-diol column using a hexane: isoproanol: trithylamine gradient. Eluates were collected on a preprogrammed fraction collector. Final quantification was achieved by RIA on the selected fractions. This procedure allowed: 1) the separation of each measured steroid from plasma contaminants; 2) precise collection; 3) quantification of mineralocorticoids with a precision and sensitivity equivalent to that of direct methods; and 4) improved specificity. © 1987 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH. 
Adrenal steroids; Glucocorticoids; HPLC-RIA; Mineralocorticoids