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HERO ID
8333639
Reference Type
Journal Article
Title
Preparative HPLC for analysis of mineralocorticoids in human plasma
Author(s)
Lejeune-Lenain, C; Kina, S; Bosson, D
Year
1987
Is Peer Reviewed?
Yes
Journal
Chromatographia
ISSN:
0009-5893
EISSN:
1612-1112
Publisher
Springer-Verlag
Location
WIESBADEN 1
Volume
24
Issue
1
Page Numbers
333-338
Language
English
DOI
10.1007/BF02688501
Web of Science Id
WOS:A1987L971400040
Abstract
A method has been developed to measure simultaneously in human plasma the mineralocorticoids and glucocorticoids involved in mineralocorticoid excess. Briefly: plasma extracts were chromatographied in a fully-automated procedure on a lichrospher-diol column using a hexane: isoproanol: trithylamine gradient. Eluates were collected on a preprogrammed fraction collector. Final quantification was achieved by RIA on the selected fractions. This procedure allowed: 1) the separation of each measured steroid from plasma contaminants; 2) precise collection; 3) quantification of mineralocorticoids with a precision and sensitivity equivalent to that of direct methods; and 4) improved specificity. © 1987 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
Keywords
Adrenal steroids; Glucocorticoids; HPLC-RIA; Mineralocorticoids
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