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8335414 
Journal Article 
Specific progesterone receptors in dimethylbenzanthracene (DMBA) induced mammary tumors 
Asselin, J; Labrie, F; Kelly, PA; Philibert, D; Raynaud, JP 
1976 
1 
Steroids
ISSN: 0039-128X
EISSN: 1878-5867 
27 
3 
395-404 
English 
Properties of a progesterone receptor present in the cytosol (105,000 xg supernatant) of dimethylbenzanthracene (DMBA) induced mammary tumors were studied using the highly potent progestin [3H]R 5020 (17, 21 dimethyl 19 nor pregna 4,9 diene 3,20 dione). As shown by sucrose gradient analysis, specific binding of [3H]R 5020 is associated with components migrating at 7-8S and 4S. Low affinity binding of the synthetic progestin is eliminated by treatment with dextran coated charcoal. [3H]R 5020 binding is highly progestin specific since it is easily displaced by unlabelled norgestrel, R 5020 and progesterone while estradiol 17β, dihydrotestosterone, testosterone and diethylstilbestrol have much lower activity. Dexamethasone and cortisol have little, if any, effect on [3H]R 5020 binding.