BESTIMMUNGSMETHODE FUR VITAMIN K1 (PHYLLOCHINON) UND MOGLICHKEITEN DER VITAMIN-K2 (MENACHINON)-BESTIMMUNG | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

8336501

Reference Type

Journal Article

Title

BESTIMMUNGSMETHODE FUR VITAMIN K1 (PHYLLOCHINON) UND MOGLICHKEITEN DER VITAMIN-K2 (MENACHINON)-BESTIMMUNG

Author(s)

Saupe, J; Shearer, MJ

Year

1992

Volume

Issue

Page Numbers

47-50

Language

German

Abstract

Phylloquinone in plasma is fat soluble. The vitamin is extracted with hexane after flocculation of proteins with ethanol. In several stages this hexane extract is purified to eliminate interfering lipids before final analysis by reversed-phase HPLC and electrochemical detection (EC). The 'coulometric' design of the EC-detector consists of two porous graphite electrodes arranged in series. The quinone is reduced at the upstream electrode and the current generated by electrolysis will be detected at the downstream electrode. Quantification is based on the method of peak height ratios after menaquinone-6 has been added to the sample as an internal standard. Menaquinones (MK-7 and MK-8) are measured in a reliable manner with the same method using external standards while MK-9 and MK-10 are seen but not measured for certain.

Keywords

HPLC-EC; menaquinones; phylloquinone