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HERO ID
8338138
Reference Type
Journal Article
Title
Determination of levalbuterol hydrochloride by HPLC
Author(s)
Wang, LL; Zheng, WS; Sun, HF
Year
2004
Is Peer Reviewed?
0
Journal
Zhongguo Yaoxue Zazhi
ISSN:
1001-2494
Volume
39
Issue
9
Page Numbers
695-696
Language
Chinese
Abstract
OBJECTIVE: To develep a simple, accurate and reproducible HPLC method for the determination of the levalbuterol hydrochloride. METHODS: Chirex(5)-ICA and (R)NEA New Column was used with N-hexane-dichloromethane-methanol- trifluotoacetic acid (240 :140:20:1) as the mobile phase. The flow rate was 1.0 mL·min-1, the UV detection wavelength was at 278 nm. RESULTS: A satisfactory separation was obtained between levalbuterol hydrochloride and related substances. The calibration curve was linear in the range of 0.004-0.8 mg·mL-1 for levalbuterol hydrochloride with good corelation coefficient (r = 0.999 8). The relative standard deviation (RSD) of intra-run and inter-run were both less than 1.5%, the determination limlngit was 0.8 ng and the quantitation limit was 2.5 ng. CONCLUSION: This HPLC assay was simple and quick with high sensitivity, and will be used for the determination of levalbuterol hydrochloride in pharmaceuticals.
Keywords
Chiral; Enantiomeric; HPLC; Levalbuterol hydrochloride
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