US EPA

8339591 

Journal Article 

Purification of soybean phosphatidylcholine by silica gel column chromatography 

Zhang, YY; Yang, YW; Ren, QL; Wu, CJ; Su, Y 

2006 

20 

5 

685-690 

Chinese 

The process of purification of phosphatidylcholine (PC) from soybean concentrated phospholipids (SCP) was studied. The method selected was based on the normal-phase Chromatography with silica gel as stationary phase and a ternary elution with n-hexane, isopropanol and water as mobile phase. Firstly, the suitable ratio of n-hexane, isopropanol and water in the mobile phase was investigated preliminarily by thin-layer Chromatography (TLC). The results show that the suitable ratio of mobile phase is n-hexane:isopropanol:water = 1:1:0.175, by volume. Secondly, the optimal operation conditions of column Chromatography separation were obtained by the investigation of the stationary phase used, mobile phase composition and column loading ability. The results show that at the sample concentration of 0.25 gSCP·(mL mobile phase)-1 and the flow rate of 0.382 cm·min-1, the 100-200 mesh silica gel can be chosen as the stationary phase, the ternary mixture of n-hexane:isopropanol:water = 1:1:0.16 (by volume) is the suitable mobile phase and the optimal loading is 0.086 gPC·(g silica gel)-1. Using above mentioned optimal operation conditions, the product purity of the column Chromatography separation at room temperature was analyzed by HPLC (High-Performance Liquid Chromatography), and the result shows that the purity of PC obtained can reach 85%. 

Column Chromatography; Phosphatidylcholine; Silica gel; Soybean concentrated phospholipids