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8346636 
Journal Article 
HPLC method for simultaneous determination of terbutaline, guaiphenesin and ambroxol in formulations 
Shenoy, KPR; Krishnamurthy, KS; Vasundhara, I 
2001 
Indian Drug Manufacturers' Association 
38 
8 
428-432 
English 
A simple, precise and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous estimation of Terbutaline Sulphate, Guaiphenesin and Ambroxol Hydrochloride in formulations. The separations were achieved on a 5-micron Waters symmetry C18 column (250 × 4.6mm) using a mobile phase consisting of a mixture of water and acetonitrile containing sodium hexane suphonate (pH 3.0). The detection of the constituents was done using UV detector at 280nm for Terbutaline Sulphate and Guaiphenesin and 250 nm Ambroxol Hydrochloride. The retention times of Terbutaline sulphate, Guaiphenesin and Ambroxol hydrochloride were approximately 3.8,6.2 and 21 minutes, respectively. Recovery study values of 98 to 102%, relative standard deviation of less than 2% for the assay, and linearity coefficient of 0.9999 show that the method is precise, accurate, and linear in the concentration given. 
Ambroxol hydrochloride; Chromatography (RPLC); Guaiphenesin; Isocratic; Reversed-phase liquid; Simultaneous determination; Terbutaline sulphate