New kinetic-spectrophotometric method for microgram determination of doxycycline hyclate in aqueous formulations, Tablets and capsules | Health & Environmental Research Online (HERO)

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Citation
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HERO ID

8351160

Reference Type

Journal Article

Title

New kinetic-spectrophotometric method for microgram determination of doxycycline hyclate in aqueous formulations, Tablets and capsules

Author(s)

Kaushik, RD; Rathi, P; Manila; Singh, J; Yadav, R

Year

2014

Is Peer Reviewed?

Journal

Journal of Chemical and Pharmaceutical Research
ISSN: 0975-7384

Volume

Issue

Page Numbers

2752-2758

Language

English

Abstract

The Mn II catalysed oxidation of Doxycycline Hyclate(DCH) by periodate ion in aqueous medium was followed by monitoring the increase in the absorbance of reaction intermediate and made the basis for developing newer method for microgram estimation of DCH. The reaction is first order with respect to substrate and oxidant each. The best fit conditions developed for a new and simple kinetic-spectrophotometric method for microgram determination of DCH in the range 51.30 μg/mL – 615.54 μg/mL are, [NaIO 4 ] = 1.0 × 10 -5 mol dm -3 , [Mn II ] = 1.0 × 10 -3 mol dm -3 , Temperature = 35.0 ± 0.1°C, pH = 5.0 and λmax = 486 nm. The characteristics of various calibration curves, percentage recovery, effect of excipients, correlation coefficient and comparison with other reported methods are presented. © 2014, Journal of Chemical and Pharmaceutical Research. All rights reserved.

Keywords

Aqueous formulations; Capsules; Doxycycline hyclate; Kinetic-spectrophotometric method; Microgram estimation; Periodate ion; Tablets