US EPA

8351277 

Journal Article 

Optimized fermentation of extracellular polysaccharides from Vitreoscilla and its antitumor activity 

Geng, XQ; Fan, BQ; Ding, QR; Du, P; Zheng, Y; Song, J 

2020 

29 

19 

2229-2238 

Chinese 

Objective: To increase the yield of extracellular polysaccharides from Vitreoscilla (VEPS) and preliminarily study its antitumor activity. Methods: Single factor experiment, Plackett-Burman test, steepest climbing path test, Box-Behnken test design and MTT were used for this study. Results: The composition and optimum ratio of the culture medium affecting the production of VEPS were determined and the antitumor activity of VEPS was studied. The optimized formula of the culture medium was as follows: peptone 5 g•L-1, lactose 39 g•L-1, potassium dihydrogen phosphate 0.17 g•L-1, dipotassium hydrogen phosphate 0.46 g•L-1, ammonium sulfate 0.2 g•L-1, and compound salt solution (magnesium sulfate 19.5 g•L-1, manganese sulfate 5 g•L-1, ferrous sulfate 1.4 g•L-1, and calcium chloride 0.05 g•L-1) 1 mL•L-1. Under this condition, the yield of extracellular polysaccharides was (583.6±6.3) mg•L-1. Compared with the initial medium, the yield increased by 364.3% with the error of predicted value 1.3%. Conclusion: In vitro anti-tumor experiments showed that VEPS had good anti-tumor activity. © 2020, Chinese Journal of New Drugs Co. Ltd. All right reserved. 

Antitumor activity; Extracellular polysaccharide; Medium optimization; Response surface design; Vitreoscilla