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8358073 
Journal Article 
The Determination of Dulcin in Foods by Useing Direct-Injecting Hydrolysis Gas Chromatography 
Ishida, H; Fujii, T; Sekine, H; Saito, Y; Sekiya, S 
1990 
1 
Shokuhin Eiseigaku Zasshi / Journal of the Food Hygienic Society of Japan
ISSN: 0015-6426
EISSN: 1882-1006 
31 
6 
479 
English 
A sample solution for the gas chromatographic analysis (GCA) of dulcin can be obtained through the following processes: 1. extraction of dulcin from a sample with water, 2. removal of fat with w-hexane after acidification, 3. extraction with ether after pH adjustment (pH 11-13) with NaOH, 4. vacuum evaporation of ether, 5. dissolving of the given residue in 98% acetonitrile (2% water content). The GCA of the acetonitrile solution was carried out by using an insert packing of KOH coated on glass wool. The conditions of GCA were as follows: a PEG 20M + KOH(5+1)% column, 3 mm X 1. 0 m; injection cavity temperature 250°C; column temperature 180°C. In the GCA, the recovery of dulcin was 95-101% and the coefficients of variation were less than 5%. The peak used for dulcin determination was identified as p-phenetidine by GC/MS. It is suggested that additional substances can be determined by GCA without derivatization, if the process of hydrolysis with packing substances in an insert is applicable. © 1990, Japanese Society for Food Hygiene and Safety. All rights reserved. 
dulcin; gas chromatography (FID); phenetidine; sweetener