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8362912 
Journal Article 
功能化石墨烯对苯并[a]芘高效降解菌Paracoccus aminovorans HPD-2生长的促进作用 
Mao, T; Ren, W; Liu, F; Teng, Y 
2020 
Yes 
Huanjing Kexue Xuebao / Acta Scientiae Circumstantiae
ISSN: 0253-2468 
Science Press 
40 
1 
251-259 
Chinese 
Studying the influence of graphene on the growth of microorganisms and deeply exploring the interaction between graphene and microorganisms are of practical significance for scientifically evaluating the ecological safety of graphene. In this paper, the effects of two functional graphene (graphene oxide and sulfonated graphene) on the growth of benzo[a]pyrene-degrading bacteria Paracoccus aminovorans HPD-2 were studied. Scanning electron microscopy (SEM), Raman spectroscopy and infrared spectroscopy technology were applied to explore the interaction mechanism between graphene and HPD-2. The results showed that the effects of both graphene on the growth of HPD-2 were related to the nutrient level in the culture system. The type and concentration of graphene were also important factors. Low concentrations of graphene (0~10 mg•L-1) had no effect on the growth of HPD-2, while higher concentration of graphene (100 mg•L-1) could significantly promote the growth of HPD-2 (p<0.05). Both types of graphene could increase the secretion of HPD-2 extracellular polymeric substance. After the interaction with HPD-2, the relative intensity ratio (ID/IG) at low concentrations of graphene oxide increased significantly, and the structural disorder increased, while the higher concentrations of graphene interacted significantly with HPD-2, and had a certain degree of stack on its surface. Proteins, amino acids and extracellular polysaccharides in cell surface were involved in the interaction. Compared to graphene oxide, the interaction of sulfonated graphene and HPD-2 surface was weaker. The results of this study are helpful to deeply understand and scientifically evaluate the microbial effects of graphene. © 2020, Science Press. All right reserved. 
Graphene oxide; Microbial growth; PAHs; Paracoccus aminovorans; Sulfonated graphene