US EPA

8363243 

Journal Article 

Blood compatibility of polyaiticglycolic acid/RNA III inhibiting peptide microspheres 

Zhang, XB; Hao, LB; Wang, JF; Yao, Q; Liang, MH 

2008 

No 

Journal of Clinical Rehabilitative Tissue Engineering Research
ISSN: 1673-8225 

12 

6 

1022-1026 

Chinese 

Aim: Studies reveal that RNAIII inhibiting peptide (RIP) is an effective suppressant for staphylococci and the mechanism obviously differs from traditional antibiotic drug, indicating its potential application. This study evaluated blood compatibility of polyaiticglycolic acid (PLGA)/RIP microspheres. Methods: The experiment was performed in the Pharmacological Research Institute and Animal Experimental Center at the General Hospital of Chinese PLA from October 2005 to October 2007. Thirty healthy adult New Zealand white rabbits were grouped randomly, with 6 animals in each group. Drugs and reagents: PLGA, dimethyl sulphoxide, MTT, DMEM, dinitoflruorobenzene.1 Preparation of PLGA/RIP microspheres: Fmoc method was used to synthesize RIP from C end to N end, then the synthesized crude peptide sample was purified by the reverse phase high performance liquid chromatography, and composition was collected by means of ultraviolet absorption peak. The purified PIR was obtained from freezing and drying. Afterwards liquid-phase multiple emulsion method was used to synthesize PLGA/RIP microspheres of 50-70 mm diameter. 2 Preparation of eluent: 1 g/L PLGA/RIP microsphere was eluted sterily with saline at 37 *C for 72 hours to harvest stock solution, then equal volume of stroke-physiological saline solution was added to obtain 0.5 g/L dilution.3 Haemolysis test: With distilled water and stroke-physiological saline solution as positive and negative controls respectively, the rate of haemolysis of 100% and 50% eluent of PLGA/RIP were studied. 4 Hemagglutinatin test and PLGA/RIP effect on prothrombin time (PT) and activated partial thromboplastin time (APTT): With stroke-physiological saline solution as negative control, the effects of 100% and 50% eluent of PLGA/RIP on PT and APTT were observed. 5 The effects of 100% and 50% eluent of PLGA/RIP on the leucocyte, erythrocyte, thrombocyte and platelet aggregation were detected. Results: Totally 30 rabbits were involved in the result analysis. 1 Haemolysis test showed that, the haemolysis rates of 100% and 50% eluent of PLGA/RIP were 3.24% and 2.67%. They were in coincidence with the criteria of biomaterials (< 5%). 2 Hemagglutinatin test showed that the 100% and 50% eluent of PLGA/RIP had no significant effect on hemagglutinatin. 3 PLG/RIP showed no significant effect on PT and APTT. 4 PLGA/RIP also showed no significant effect on leucocyte, erythrocyte and thrombocyte. 5 PLGA/RIP showed no significant effect on platelet aggregation. Conclusion: PLGA/RIP microspheres have good blood compatibility.