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8202810 
Journal Article 
TGF-β suppresses the expression of genes related to mitochondrial function in lung A549 cells 
Sohn, EJ; Kim, J; Hwang, Y; Im, S; Moon, Y; Kang, DM; , 
2012 
Yes 
Cellular and Molecular Biology
ISSN: 0145-5680
EISSN: 1165-158X 
Suppl.58 
OL1763-OL1767 
English 
23046869 
TGF-β is a mediator of lung fibrosis and regulates the alveolar epithelial type II cell phenotype. TGF-β can induce epithelial mesenchymal transition of idiopathic pulmonary disease and cancer metastasis. Peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC-1 α) is a key metabolic regulator that stimulates mitochondrial biogenesis and promotes remodeling of muscle tissue to oxidative fiber-type composition. Here, we report that the induction of TGF-β decreased mRNA expression of PGC-1α, and PGC-1 target genes, such as the transcription factors NRF-2, ERR-α, and PPAR-γ in lung epithelial A549 cells. In addition, TGF-β led to the reduction of super oxide dismutase 2 (anti-oxidant enzyme), cytochrome C (electron transport chain in mitochondria), and MCAD (a mitochondrial β-oxidant enzyme) in A549 cells. Together, our results suggest that TGF-β may suppress the transcriptional activity of the genes related to mitochondrial biogenesis or function. This mechanism may provide a novel insight into the understanding of fibrosis disease. 
Cell Line, Tumor; Cytochromes c/genetics/metabolism; Gene Expression Regulation; Heat-Shock Proteins/genetics/metabolism; Mitochondria/metabolism; NF-E2-Related Factor 2/genetics/metabolism; PPAR gamma/genetics/metabolism; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Receptors, Estrogen/genetics/metabolism; Superoxide Dismutase/genetics/metabolism; Transcription Factors/genetics/metabolism; Transforming Growth Factor beta/pharmacology