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8406312 
Journal Article 
Fluorometric determination of non-fluorescent amines by ion-pair extraction 
Borg, KO; Westerlund, D 
1970 
0 
Fresenius' Zeitschrift für Analytische Chemie
ISSN: 0016-1152 
Springer-Verlag 
252 
4-5 
275-278 
English 
An ion pair extraction method is described for quantitative fluorometric determination in the ng/ml range of non-fluorescent amines used as drugs. As counter ion, reagent, anthracene-2-sulphonate was used. Its extracting ability of ion pairs with amines was studied by determination of extraction constants and its fluorescence properties by determination of quantum yield of fluorescence. The properties of a suitable organic solvent is discussed and methylene chloride was found to be advantageous. Conditions for quantitative extraction and determination are given and the method was applied to determination of amitriptyline in the range 10-100 ng/ml giving an extraction degree of 100% and a precision of 5%. © 1970 Springer-Verlag.