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84084 
Journal Article 
Manganese-induced oxidative stress as measured by a fluorescent probe: an in vitro study 
Soliman, EF; Slikker, W, Jr; Ali, SF 
1995 
1 
Neuroscience Research Communications
ISSN: 0893-6609
EISSN: 1520-6769 
17 
3 
185-193 
English 
WOS:A1995TJ20700005 
The fluorescent probe, 2',7'dichlorofluorescein-diacetate (DCFH-DA) was used to quantitate the formation of reactive oxygen species (ROS) in brain tissue. Sprague-Dawley rats were sacrificed at different ages, postnatal day (PND) 1, 24, 41 and 4 and 18 months, and brains were dissected into caudate nucleus (CN) and cerebellum (CE). In vitro exposure to Mn (0.2-2.0 mM) increased the formation of ROS in brain synaptosomes at all ages. Age-related differences were found in the formation of ROS between CN and CE. In PND 1 brain synaptosomes, Mn induced dose dependent (0.2-2.0 mM) increases in the formation of ROS. This effect was also observed at other ages in CN and CE, but at higher concentrations (0.8-2.0 mM). It may be concluded that oxidative stress, as measured by ROS, may be a potential mechanism underlying the neurotoxicity induced by Mn and that the neonatal rat brain may be more susceptible than the adult rat brain. 
MANGANESE; REACTIVE OXYGEN SPECIES; OXIDATIVE STRESS; AGING; DICHLOROFLUORESCEIN; NEUROTOXICITY