US EPA

8409013 

Journal Article 

Antimicrobial, cytotoxic and antioxidant activity of Centella asiatica 

Ullah, MO; Sultana, S; Haque, A; Tasmin, S 

2009 

EuroJournals, Inc. 

30 

2 

260-264 

English 

The n-hexane, carbon tetrachloride, chloroform soluble fractions of methanol extract from the plant Centella asiatica (Apiaceae) was subjected to antioxidant, antimicrobial and brine shrimp lethality bioassay. All the fractions showed moderate to potent antioxidant activity, of which the chloroform and aqueous soluble fraction demonstrated the strongest antioxidant activity with the IC50 value of 4.0 μg/ml and 7.0 μg/ml, respectively. In case of antimicrobial screening, crude extracts showed notable antibacterial and antifungal activity against sixteen microorganisms. The n-hexane, carbontetrachloride, chloroform and aqueous soluble partitionates of the methanoic soluble fractions showed average zone of inhibition ranged from 7-15 mm, 8-12 mm, 8-16 mm and 8-13 mm, respectively, at a concentration of 400 μg/disc. However, in the brine shrimp lethality bioassay, all the crude extracts possessed considerable cytotoxic activity. It was evident that, the n-hexane, carbon tetrachloride, chloroform and aqueous soluble fractions have significant cytotoxic potentials having LC50 1.254, 0.826, 3.866 and 5.366μg/ml respectively. © EuroJournals Publishing, Inc. 2009. 

Apiaceae; Brine shrimp; Disc diffusion; Free radical scavenging