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8409798 
Journal Article 
High performance thin layer chromatographic method for the determination of piperine from piper nigrum linn 
Kulkarni, D; Apte, SP; Mary, F; Sane, RT 
2001 
Indian Drug Manufacturers' Association 
38 
6 
323-326 
English 
Piper nigrum commonly known as black pepper is used as spice as well as medicine. Its use in dyspepsia, flatulence and cholera is very frequent. The active principle of black pepper, piperine, which has therapeutic properties and acts as a bioavailability enhancer. The present work on quantitative determination of piperine was to provide a easy and simple analytical method, which can be used as a routine quality control method. Samples from a market were authenticated and used for analysis. High performance thin layer chromatography was performed on silica gel 60 F254 HPTLC plates. The mobile phase was n-hexane: ethyl acetate (7:3 v/v). The detection and quantification was performed at a wavelength 340 nm. Linearity of detector response for piperine was 20 ng to 100 ng. The correlation coefficient obtained for the linearity was 0.994. The standard error of the Y estimate was 133.2405 and standard error of the X coefficient 0.67994. The mean assay value of piperine was found to be 50.78 mg/gm of pepper. The recovery value of piperine was 100.27%. Low values of standard deviation and % C.O.V. are indicative of the high precision of the method. 
Black pepper; Densitometry; HPTLC - silica gel; Piperine