US EPA

8410854 

Journal Article 

A comparative study on the antioxidant activity of methanol extracts of acacia nilotica and berberis chitria 

Agrawal, S; Kulkarni, GT; Sharma, VN 

2010 

4 

1 

78-84 

English 

In this study, antioxidant activity of methanol extract of Acacia nilotica (AN) and Berberis chitria (BC) were evaluated using different in vitro methods. ABTS, DPPH, Nitric oxide, hydroxyl radical and hydrogen peroxide scavenging activities were used as standard methods of evaluation of antioxidant properties. Total phenolic content of the selected extracts was estimated by Folin-Ciocalteu method and correlated with antioxidant properties of the extracts. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and Ascorbic acid (ASC) were used as the reference antioxidant compounds. Both the extracts were found to possess significant antioxidant property. Among the two, Acacia nioltica had higher antioxidant property than Berberis chitria. The antioxidant property was directly related to the total phenolic content of the extract, which was found to be 9.88 and 2.73 μg/ml, respectively for AN and BC. From the above studies it can be suggested that Acacia nilotica could be used as an antioxidant, probably for the treatment of diseases related to free radicals, such as, cancer, diabetes, etc. Further studies are needed on the isolation and identification of antioxidant compounds in AN to prove its worth as antioxidant drug. © 2010, American Eurasian Network for Scientific Information. 

Acacia nilotica; Antioxidant; Berberis chitria; Free radical scavenging