Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
8414974
Reference Type
Journal Article
Title
Protein purification: Alcohol Washing of Soybean Protein
Author(s)
Eldridge, AC; Wolf, WJ; Nash, AM; Smith, AK
Year
1963
Is Peer Reviewed?
Yes
Journal
Journal of Agricultural and Food Chemistry
ISSN:
0021-8561
EISSN:
1520-5118
Volume
11
Issue
4
Page Numbers
323-328
Language
English
DOI
10.1021/jf60128a015
Abstract
Soybean protein isolated by acidification of an aqueous extract of hexane-defatted soybean meal contains 2 to 4% of a phospholipide-like material. These lipides cannot be removed by isoelectric precipitation, washing with water, dialysis, or ammonium sulfate precipitation. However, they are extractable with aqueous alcohols. The concentration of alcohol used to wash the protein has a pronounced effect on the amount of material removed and on the nitrogen content of the extracted protein. Optimum concentrations of alcohol are: methyl, 95 to 97% (v./v.); ethyl, 84 to 88% (v./v.); and isopropyl, 78 to 88% (v./v.). The effects of extraction time, temperature, pH, and solvent to protein ratio were also studied. The washed protein has improved color and flavor and produces extremely stable, low density foams and whips similar to egg white and commercially available soy products. © 1963, American Chemical Society. All rights reserved.
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity