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8416838 
Journal Article 
Gas Chromatography of Ubiquinone and Related Quinones 
Morimoto, H; Imada, I 
1971 
1 
Methods in Enzymology
ISSN: 0076-6879
EISSN: 1557-7988 
Methods in Enzymology 
18 
169-179 
English 
This chapter discusses a gas chromatographic procedure for the extraction of ubiquinone (Q) and related quinines. The analysis of Q is usually carried out by the ultraviolet method on each homolog. When several homologs coexist, it is advantageous to apply a gas chromatographic procedure that does not comprise a separation for each homolog. For concentration, the lipid fraction is extracted and the Q fraction is separated from the other lipids. Methods that may possibly lead to some alterations in Q, such as alkaline hydrolysis, should not be used in the course of concentration, and it is preferable to obtain the Q fraction by simple solvent extraction and chromatography. For extraction of lipid from natural material without any loss of Q, the original material is treated with a hydrophilic organic solvent, such as a lower alcohol, with warming, and the extract is further treated with a lipophilic solvent, such as hexane. A lipid fraction is obtained by evaporation of the extract. © 1971, Academic Press, Inc.