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8418105 
Journal Article 
Metabolic activation of mutagens in mammals host-mediated assay utilizing the induction of mitotic gene conversion in Saccharomyces cerevisiae 
Fahrig, R; , 
1973 
1 
Agents and Actions
ISSN: 0065-4299 
Birkhäuser-Verlag 
3 
2 
99-110 
English 
4580186 
It was demonstrated the genetic activity of 10 mutagens in the host-mediated assay in comparison with treatment of yeast cells in vitro. 4-Nitroquinoline 1-oxide (4-NQO) 2-Methoxy-6-chloro-9-(2-chloroethylaminopropylamino) acridine dihydrochloride (ICR-191) N-Methyl-N-nitro-N-nitrosoguanidine (MNNG) Methyl methanesulfonate (MMS) Ethyl methanesulfonate (EMS) 1-Phenyl-3,3-dimethyltriazene (PDT) 1-(Pyridyl-3)-3,3-dimethyltriazene (PyDT) 1-(Pyridyl-3-N-oxide)-3,3-dimethyltriazene (PyNDT) 2-[Bis-(chlorethyl-)amino]-tetrahydro-2 H-1,3,2-ozaxaphosphorine-2-oxide (Endoxan) Dimethylnitrosamine (DMNA) Five of these substances-PDT, PyDT, PyNDT, Endoxan and DMNA-proved to be metabolically activated in the mouse. With two substance-4-NQO and MMS-a more thorough study was conducted, illustrating the effect of dose and time on the induction of gene conversion. Comparing the dose effect and time effect curves resulting from the in vitro test and the host-mediated assay, the general observation that could be made was that dose effect and time effect curves obtained from microbial test systems, seem to have little or no relevance for man. The relationship of dose and time to induction of gene conversion in vitro was consistently an exponential one whereas in the host-mediated assay each substance had its own characteristic dose/effect and time/effect relationship. © 1973 Birkhäuser-Verlag.