Kumar, V; Singh, S; Kondalkar, SA; Srivastava, B; Sisodia, BS; Barthi, B; Singh, R; Prakash, O
Plants and their secondary molecules are considered as unique gift of God to humans. Secondary molecules of plants are considered as major therapeutic agents to cure diverse classes of disease. The aim of the present study was to identify the secondary molecules of the leaf extract of Holoptelea integrifolia (HI). Identification of biomolecules using the fourier transformation infrared (FTIR) and gas chromatography coupled with high resolution mass spectrometer (GC-HRMS) have confirmed the presence of seven prominent molecules including four new molecules. The four new molecules identified though GC-HRMS were (Z)-octadeca-9,17-dienal (13.17%), (9Z,12Z)-octadeca-9,12-dienoyl chloride (3.22%), 1,3-dihydroxypropan-2-yl palmitate (6.01%), and 17-acetyl-14-hydroxy-10,13-dimethyl-decahydro-2H-cyclopenta[α]phenanthrene-3,12,15(4H,14H,16H) - trione. The aqueous extract of HI leaves exhibited the antioxidant potential (>59%), anti-diabetic (78.45 ± 5.55%), anti-inflammatory (value of binding constants (k) was 1.83 ± 0.06 à 10â4 μMâ1) and antimicrobial (MIC values 20â30 mg/ml) qualities. Moreover, the observed values of various physicochemical parameters including pH, loss on drying, total ash, acid insoluble ash, water extractive values, and alcohol extractive studies were 7.07 ± 0.37, 8.55 ± 0.75%, 18.12 ± 1.21%, 10.07 ± 0.87%, 37.97 ± 2.31%, and 29.36 ± 1.27% respectively. Also, pharmacognostic analyses were performed. The results of present study could contribute to the isolation of new compounds those may have medicinal qualities. © 2019 Elsevier Ltd