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8428329 
Journal Article 
Lipidextraktionen mit Hilfe von Extrelut zur gas- und dünnschicht-chromatographischen Analyse von Triglyceriden, Cholesterinestern und Fettsäuren aus Serum und Milch 
Klump, B; Melchert, HU; Rubach, K 
1982 
0 
Fresenius' Zeitschrift für Analytische Chemie
ISSN: 0016-1152 
Springer-Verlag 
313 
7 
553-560 
German 
A method is described for isolating all main lipid classes from native serum and milk samples with the aid of Extrelut (cholesterol and its esters, mono-, di- and triglycerides, phospholipids and free fatty acids). A sample volume of 50 μl is sufficient to obtain lipid extracts by means of an Extrelut extraction for TLC- or GLC-patterns of total lipids. Furthermore, Extrelut is also useful for the extraction of larger amounts of sample material. For the preparation of total lipid extracts for TLC-analyses the crude material is given onto a small column filled with Extrelut and is extracted with the solvent mixture hexane/isopropanol (3/2; v/v). Phospholipid-free extracts for GLC-analyses (glass columns of 55cm length and 2mm i.d. 1% Dexsil 300GC on Supelcoport 100/120 mesh) are achieved by means of an Extrelut extraction with hexane after on-column-destruction of the lipoproteins with methanol. In the case of the analysis of individual lipid classes TLC-fractionation of the Extrelut extracts can easily be performed. This method of isolating lipids is not only comparable to conventional extraction methods but also exhibits some remarkable advantages. Formation of emulsions and interfering intermediates is totally avoided using Extrelut and an easy time-saving work is favoured by application of small solvent volumes. The need of very small sample amounts allows to perform micro-scale analyses with high accuracy. Multi-stage elutions with solvents or solvent mixtures of different polarity facilitate the feasibility of fractionated lipid extractions. © 1982 Springer-Verlag.