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8429064 
Journal Article 
Estimation of pentoses and methylpentoses in biopolymers, in particular of fucose and xylose 
Tsiganos, CP; Muir, H 
1966 
Yes 
Analytical Biochemistry
ISSN: 0003-2697
EISSN: 1096-0309 
17 
3 
495-501 
English 
1. (1) Conditions under which pentoses and methylpentoses react with anthrone in sulfuric acid have been reexamined. 2. (2) The number of steps involved has been reduced and good reproducibility obtained. 3. (3) Xylose was the most reactive pentose; other pentoses and methylpentoses required longer times of heating at 40°C. These differences in reaction rates and absorption spectra were utilized to estimate fucose and xylose in a mixture. 4. (4) Interference due to hexoses, hexuronic acids, hexosamine, sialic acid, and deoxyribose was virtually eliminated and no interference by tryptophan and protein was observed. 5. (5) Bound fucose and xylose reacted as easily as the free sugars. Although ribose in RNA did not, nevertheless the method could be used to estimate RNA in the presence of DNA provided an RNA standard is used. © 1966.