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8430835 
Journal Article 
Sterilization of Leukemic Cells in Vivo and in Vitro 
Burchenal, JH; Cremer, MA; Williams, BS; Armstrong, RA 
1951 
Yes 
Cancer Research
ISSN: 0008-5472
EISSN: 1538-7445 
11 
9 
700-705 
English 
1. An in vivo technic for inactivating leukemic cells has been described as an aid in differentiating between modes of action of compounds chemotherapeutically active against transplanted mouse leukemia. 2. Six nitrogen mustards and two ethyleni-mines have been tested by this method against strains Ak4, Ak 9417, and Ak 1394 of mouse leukemia. With the exception of 2-ethylenimino-4,6-dimethoxy-s-triazine, all showed a definite sterilizing effect on leukemic cells. 3. 2,6-Diaminopurine and five antagonists of pteroylglutamic acid were tested and found to have no inactivating properties in vivo at dosage levels up to 50 and 100 times the LD50. 4. Even when nephrectomized mice were used in an attempt to reduce the excretion of the drug, massive doses of 4-amino-N10-methyl-PGA did not sterilize the leukemic cells in vivo. 5. An in vitro technic was also described in which methylbis (2-chlorethyl) amine and 2,6-di-aminopurine sterilized leukemic cells at concentrations corresponding to 1–4 times the LD50 dose for the intact animal. By this method, however, 4-amino-N10-methyl-PGA had no effect even at concentrations corresponding to 50–100 times the ld50dose. © 1951, American Association for Cancer Research. All rights reserved.