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8449728 
Journal Article 
Simultaneous determination of corticosteroids and parabens in ointments by HPLC 
Maeda, Y; Owada, K; Yamamoto, M; Sano, S; Masui, T; Nakazawa, H 
1988 
1 
Bunseki Kagaku / Analysis Chemistry
ISSN: 0525-1931 
37 
12 
648-653 
English 
A rapid, simple and reliable method was developed for the simultaneous determination of eleven corticosteroids, such as prednisolone, triamcinolone acetonide, fluocinolone acetonide, hydrocortisone butyrate, fluo-cinonide, difluprednate, dexamethasone valerate, betamethasone valerate, prednisolone valerate-acetate, beclomethasone dipropionate and diflucortolone valerate, and four parabens, such as methyl, ethyl, n-propyl and n-buthylparaben in ointments by HPLC. The method involves a clean-up on a disposable Sep-Pak silicagel cartridge. Fat-soluble excipients are removed through the cartridges with 10 ml of hexane-chloroform (70:30). Corticosteroids and parabens are then eluted from the cartridges with 10 ml of ethyl acetate and determined by HPLC on a reversed-phase column with water-methanol (50 : 50 and 60 : 40) as the mobile phase, and detected at 240 nm with 0.04AUFS using s-butylparaben as an internal standard. The method was successfully applied to the analysis of ointement base spiked corticosteroids and parabens, and the sufficient recoveries (98.4~103.2%) were obtained with relative standard deviation of 0.4~2.4%. © 1988, The Japan Society for Analytical Chemistry. All rights reserved. 
pretreatment with disposable silicagel cartridge; reversed-phase HPLC; simultaneous determination of corticosteroids and parabens in ointment