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8450565 
Journal Article 
Determination of epigoitrin and goitrin in isatidis radix by chiral high performance liquid chromatography 
Nie, L; Wang, G; Dai, Z; Lin, R 
2010 
1 
Sepu / Chinese Journal of Chromatography
ISSN: 1000-8713
EISSN: 1872-2059 
28 
10 
1001-1004 
Chinese 
A Chiralpak IC column (250 mm x 4. 6 mm, 5|xm) was used to establish normal phase high performance liquid chromatographic (NP-HPLC) method for the chiral separation of epigoitrin (R-goitrin) and goitrin (S-goitrin) in Isatidis Radix. The composition of mobile phase, flow rate and column temperature were optimized to get better resolution. The final chromatographic conditions were established as follows1 w-hexane-isopropanol (90:10, v/v) as the mobile phase with a flow rate of 0. 8 mL/min, the detection wavelength at 245 nm and the column temperature at 20oC. Under the above optimized chromatographic conditions, the resolution of 3. 4 was achieved for epigoitrin and goitrin. The limit of detection (LOD) was 2. 0 mg/L. The linear range was 0. 02 -2. 0 g/L. The average recovery was 101% with the relative standard deviation CRSD) lower than 3.0% (n =6). The proposed method was exclusive by determining the antivirus epigoitrin and its enantiomer separately; which provided the effective quality control of Isatidis Radix. 
Chiral high performance liquid chromatography; Epigoitrin (R-goitrin); Goitrin (S-goitrin); Isatidis Radix