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8470873 
Journal Article 
Measurement of oleic acid in human plasma by isotope dilution mass spectrometry 
Susanto, F; Humfeld, S; Reinauer, H 
1986 
Yes 
Chromatographia
ISSN: 0009-5893
EISSN: 1612-1112 
Springer-Verlag 
21 
12 
693-696 
English 
A method for the quantitative determination of oleic acid in human plasma by isotope-dilution mass spectrometric technique is described. For the measurement of the fatty acid concentration (1-13C) oleic acid is added to the plasma sample. The fatty acids are extracted with n-hexane. Portions of the extract are esterified by the boron trifluoridemethanol method or stable isotope methylation. The methyl ester derivatives of the fatty acids are separated and detected by GC-MS with the mass spectrometer set to m/z 296 and 297. For the measurement of oleic acid after stable isotope methylation the m/z 299 and m/z 300 are monitored. The amounts of oleic acid in the plasma are calculated from the isotope ratios measured by selected ion monitoring. The recovery of the methylation step and the precision and accuracy of the GC-MS method are presented and discussed. © 1986 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH. 
Gas chromatography/mass spectrometry; Isotope-dilution mass spectrometry; Methylation methods; Oleic acid in plasma