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8471130 
Journal Article 
High speed liquid chromatographic analysis of fat soluble tar dyes 
Ohnishi, S; Nishijima, Y; Kijima, K; Kano, S 
1977 
1 
Bunseki Kagaku / Analysis Chemistry
ISSN: 0525-1931 
26 
12 
814-818 
English 
Fat soluble tar dyes for cosmetic products (C.I. No.12120, 26100, 12085, 12075, 12315, 26105, 12140, 12100, 11680, 11380, 11390 and 47000) were separated on a LiChrosorb SI 100 (5μ, irregularly shaped porous silica gel) column by the isocratic elution technique. The experiment was carried out using a model 635 liquid chromatograph (Hitachi Ltd.) equipped with wavelength tunable effluent monitor. The high speed liquid chromatographic condition was as follows; column size: 4mm i.d.× 250 mm, detector: VIS spectrophotometer {(420~500) nm}, flow rate: 1.5 ml/min. Eight tar dyes except C.I. No. 12140, 12100, 12075 and 12315 were separated with chloroform and n-hexane mixtures as eluent. C.I. No. 12140 and 12100 were separated with diethylether/n-hexane=4/96 mixture and C.I. No. 12075 and 12315 were separated with acetone/n-hexane =9/91 mixture as eluents, respectively. These methods were suitable for the quantitative analysis. The plots of peak height vs. amount of tar dyes were linear with the injection volume of 5 microliters or less without a lowering of column efficiency. The recoveries from wax base of lip sticks ranged from 91.9% to 97.0%. Permanent orange was only detected from commercial lip sticks and its contents were (0.0841.69)%. © 1977, The Japan Society for Analytical Chemistry. All rights reserved. 
Adsorption chromatography; Cosmetic product; Fat soluble tar dye; High speed liquid chromatography