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8506675 
Journal Article 
Pine Bark Constituents Related to Feeding Response by Japanese Sawyer Adult, Monochamus alternatus Hope (I) Bioassays in Small Vessels and Some Properties of the Biting Factors 
Miyazaki, M; Oda, K; Yamaguchi, A; Yamane, A; Enda, N 
1974 
56 
7 
239-246 
English 
Observations on feeding incitants distributed in the bark of host pine trees by newly emerged Monochamus alternatus adults are reported in the present paper. Bioassays were conducted in circular or rectangular plastic vessels containing filter paper pre-soaked in solutions of bark extractives or water as control and the adult was released (Photo. 1 and 2). After forty-eight hours or more, feeding responses were checked by counting the biting scars on the paper. The results are as follows: 1. In comparison of feeding responses to water extractives from leaves of Pinus thunbergii with those from the bark of current and one year-old twigs, more intensive responses were observed on the bark extractives (Photo. 4). Further, relatively more intensive responses occurred on acetone extractives from the one year twig bark than on those from the current year twig bark (Photo. 5). 2. Among the responses to methanol soluble materials prepared from hot water extractives from current, one and two year twig bark and trunk bark of Pinus densiflora, the most intensive response was also shown on the one year twig bark extractives, and rather reduced feeding was observed on the extractives from the others (Photo. 6). 3. Hot water extractives from current and one year twig bark of Pinus densiflora were subjected to bioassay in a cage and observations similar to those in small vessels were obtained (Photo. 3 and 8). 4. Most ot the chemical constituents related to biting tactors and feeding stimulants in the bark were easily soluble in water, acetone and methanol (Photo. 9), but slightly soluble or insoluble in non-polar solvents such as benzene and n-hexane. From bioassays on paper chromatograms (Photo. 10), the constituents were considered to be fairly polar materials. 5. Biting factor and feeding stimulant materials were unvolatile under the condition of usual steam distillation and remained in the distillation residue (Photo. 11). 6. Combination of the column chromatographys and the bioassays (Photo. 12 a and 12 b) suggests that the extractives contain at least three feeding active constituents to the adults. © 1974, THE JAPANESE FORESTRY SOCIETY. All rights reserved.